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dc.contributor.authorLuptáková, Dominika
dc.contributor.authorPluháček, Tomáš
dc.contributor.authorPetřík, Miloš
dc.contributor.authorNovák, Jiří
dc.contributor.authorPalyzová, Andrea
dc.contributor.authorSokolová, Lucie
dc.contributor.authorŠkríba, Anton
dc.contributor.authorŠedivá, Blanka
dc.contributor.authorLemr, Karel
dc.contributor.authorHavlíček, Vladimír
dc.date.accessioned2018-04-04T09:03:51Z-
dc.date.available2018-04-04T09:03:51Z-
dc.date.issued2017
dc.identifier.citationLUPTÁKOVÁ, D., PLUHÁČEK, T., PETŘÍK, M., NOVÁK, J., PALYZOVÁ, A., SOKOLOVÁ, L., ŠKRÍBA, A., ŠEDIVÁ, B., LEMR, K., HAVLÍČEK, V. Non-invasive and invasive diagnoses of aspergillosis in a rat model by mass spectrometry. Scientific Reports, 2017, roč. 7, č. NOV, s. 1-10. ISSN: 2045-2322en
dc.identifier.issn2045-2322
dc.identifier.uri2-s2.0-85036560721
dc.identifier.urihttp://hdl.handle.net/11025/29485
dc.format10 s.cs
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rights© Nature Publishing Groupen
dc.titleNon-invasive and invasive diagnoses of aspergillosis in a rat model by mass spectrometryen
dc.typečlánekcs
dc.typearticleen
dc.rights.accessopenAccessen
dc.type.versionpublishedVersionen
dc.description.abstract-translatedInvasive pulmonary aspergillosis results in 450,000 deaths per year and complicates cancer chemotherapy, transplantations and the treatment of other immunosuppressed patients. Using a rat model of experimental aspergillosis, the fungal siderophores ferricrocin and triacetylfusarinine C were identified as markers of aspergillosis and quantified in urine, serum and lung tissues. Biomarkers were analyzed by matrix-assisted laser desorption ionization (MALDI) and electrospray ionization mass spectrometry using a 12T SolariX Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. The limits of detection of the ferri-forms of triacetylfusarinine C and ferricrocin in the rat serum were 0.28 and 0.36 ng/mL, respectively. In the rat urine the respective limits of detection achieved 0.02 and 0.03 ng/mL. In the sera of infected animals, triacetylfusarinine C was not detected but ferricrocin concentration fluctuated in the 3-32 ng/mL range. Notably, the mean concentrations of triacetylfusarinine C and ferricrocin in the rat urine were 0.37 and 0.63 μg/mL, respectively. The MALDI FTICR mass spectrometry imaging illustrated the actual microbial ferricrocin distribution in the lung tissues and resolved the false-positive results obtained by the light microscopy and histological staining. Ferricrocin and triacetylfusarinine C detection in urine represents an innovative non-invasive indication of Aspergillus infection in a host.en
dc.subject.translatedFUMIGATUS,SIDEROPHORES,GA-68-SIDEROPHORESen
dc.identifier.doi10.1038/s41598-017-16648-z
dc.type.statusPeer-revieweden
dc.identifier.document-number416398300003
dc.identifier.obd43920591
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Články / Articles (KMA)
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