Title: Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen
Authors: Kostelanská, Marie
Freisleben, Jaroslav
Bačkovská Hanusová, Zdeňka
Moško, Tibor
Vik, Robert
Moravcová, Daniela
Hamáček, Aleš
Mosinger, Jiří
Holada, Karel
Citation: KOSTELANSKÁ, M., FREISLEBEN, J., BAČKOVSKÁ HANUSOVÁ, Z., MOŠKO, T., VIK, R., MORAVCOVÁ, D., HAMÁČEK, A., MOSINGER, J., HOLADA, K. Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen. Journal of Biophotonics, 2019, roč. 12, č. 8, s. 1-13. ISSN 1864-063X.
Issue Date: 2019
Publisher: Wiley
Document type: článek
URI: 2-s2.0-85065342147
ISSN: 1864-063X
Keywords in different language: decontamination;photodynamic inactivation;phthalocyanines;prions;singlet oxygen
Abstract in different language: Prion disorders are fatal neurodegenerative diseases caused by the autocatalytic conversion of a natively occurring prion protein (PrPC) into its misfolded infectious form (PrPTSE). The proven resistance of PrPTSE to common disinfection procedures increases the risk of prion transmission in medical settings. Herein, we present the effective photodynamic inactivation (PDI) of prions by disulfonated hydroxyaluminum phthalocyanine (AlPcOH(SO3)2) utilizing two custom‐built red light sources. The treatment eliminates PrPTSE signal in infectious mouse brain homogenate with efficiency that depends on light intensity but has a low effect on the overall protein content. Importantly, singlet oxygen (O2(1Δg)) is the only species significantly photogenerated by AlPcOH(SO3)2, and it is responsible for the PDI of prions. More intensive light conditions show not only higher O2(1Δg) production but also decreases in AlPcOH(SO3)2 photostability. Our findings suggest that PDI by AlPcOH(SO3)2‐generated O2(1Δg) represents a promising approach for prion inactivation that may be useful in future decontamination strategies for delicate medical tools.
Rights: Plný text je přístupný v rámci univerzity přihlášeným uživatelům.
© Wiley
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