Title: | Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen |
Authors: | Kostelanská, Marie Freisleben, Jaroslav Bačkovská Hanusová, Zdeňka Moško, Tibor Vik, Robert Moravcová, Daniela Hamáček, Aleš Mosinger, Jiří Holada, Karel |
Citation: | KOSTELANSKÁ, M., FREISLEBEN, J., BAČKOVSKÁ HANUSOVÁ, Z., MOŠKO, T., VIK, R., MORAVCOVÁ, D., HAMÁČEK, A., MOSINGER, J., HOLADA, K. Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen. Journal of Biophotonics, 2019, roč. 12, č. 8, s. 1-13. ISSN 1864-063X. |
Issue Date: | 2019 |
Publisher: | Wiley |
Document type: | článek article |
URI: | 2-s2.0-85065342147 http://hdl.handle.net/11025/35696 |
ISSN: | 1864-063X |
Keywords in different language: | decontamination;photodynamic inactivation;phthalocyanines;prions;singlet oxygen |
Abstract in different language: | Prion disorders are fatal neurodegenerative diseases caused by the autocatalytic conversion of a natively occurring prion protein (PrPC) into its misfolded infectious form (PrPTSE). The proven resistance of PrPTSE to common disinfection procedures increases the risk of prion transmission in medical settings. Herein, we present the effective photodynamic inactivation (PDI) of prions by disulfonated hydroxyaluminum phthalocyanine (AlPcOH(SO3)2) utilizing two custom‐built red light sources. The treatment eliminates PrPTSE signal in infectious mouse brain homogenate with efficiency that depends on light intensity but has a low effect on the overall protein content. Importantly, singlet oxygen (O2(1Δg)) is the only species significantly photogenerated by AlPcOH(SO3)2, and it is responsible for the PDI of prions. More intensive light conditions show not only higher O2(1Δg) production but also decreases in AlPcOH(SO3)2 photostability. Our findings suggest that PDI by AlPcOH(SO3)2‐generated O2(1Δg) represents a promising approach for prion inactivation that may be useful in future decontamination strategies for delicate medical tools. |
Rights: | Plný text je přístupný v rámci univerzity přihlášeným uživatelům. © Wiley |
Appears in Collections: | Články / Articles (RICE) Články / Articles (KET) OBD |
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